gp70 blocking. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. SpectraMax Multi-Mode Microplate Readers. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. 94 g calcium chloride dihydrate (both. MCE has not independently confirmed the accuracy of these methods. 0, MSD) or Spectramax 340 (Molecular Devices). -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Data were expressed as the mean percent viable cell vs. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The VersaMax and SpectraMax 190 read 96-well microplates. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. Assay setup for a 384-well low-volume plate. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. StakMax. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Detection limits. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). The initial rate of hydrolysis was determined using the early linear region of. The growth. SpectraMAX 340 plate reader (Molecular Devices). Clotting times (mean ± SD) for rSimukunin and rSV. 1 mL of culture media with 10% FBS. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). Cet appareil ISM est confomre à la norme NMB-001 du Canada. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax Multi-Mode Microplate Readers. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. Vessel Sprout Formation Assay. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . Male 8 pin mini Din to Female 9D. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). The reaction was stopped with 1 M hydrochloric acid after 15 min. The cell survival. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). Briefly, cells were sequentially treated with terminal deoxynucleotidyl. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. Each experiment was performed in duplicate and repeated at. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. ac. SpectraMax Multi-Mode Microplate Readers. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 6. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. The absorbances were transformed to velocity of substrate consumption (nmol/min). Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Lactate Dehydrogenase (LDH) Release. ), which was proportional to the number of damaged cells. Isolation of RNA and RT-PCR. Immunofluorescence and Aggregation Analysis. View the Molecular Devices website. SpectraMax® Microplate Readers and SoftMax® Pro Software. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. SPECTRAmax® 190. SpectraMAX 340. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. 6. The concentration of TNFα in the experimental. The chamber contains. or microplate reader (e. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. ). Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). The ABS reader ranges from. Incubation with TMB substrate was done for 18 min. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. 2. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. 3 0. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. , Sunnyvale, CA, USA) at 595 nm. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 4. Molecular Devices SpectraMax M5e Microplate Reader. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. 0 3. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. . Both readers feature an 8-channel read head for fast reads and. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The VersaMax and SpectraMax 190 read 96-well microplates. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. 5% phosphoric acid) in microplates at room temperature for 10 min. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. plates can be used in the SPECTRAmax PLUS 384. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. SPECTRAMAX 340. SpectraMax 340 from Molecular Devices). Calculating % of total mediator release in RBL assay. One plate carrier adapter is provided with the instrument. The SPECTRAmax 340PC can measure. Introduction. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. Molecular Devices SpectraMAX 340 Microplate Reader. TGFα release was calculated as described in the Results. Stat Fax 4700 Microstrip Reader. 0% and ± 0. Acceptor filter: 665/10 nm. Bertin Precellys Evolution Touch Homogenizer. The microtiter plate was then incubated in the dark for 2 h at 37°C. 2. 312 gain calibration failed. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. 5. These isotypes cause antigen cross-linking by multimeric binding and self. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Page: 1/6. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. 00 USD. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Number of flashes. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). #N207) LAL Reagent Water (Lonza cat. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Emission. The Catalina. Samples containing 25 μg of protein were separated on 12. 303 unable to cal dark current. This test was performed in duplicates. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Wells were washed three times. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Male 8 pin mini Din to Female 9D. 5), L-ascorbic acid (25. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Assay setup for a 384-well low-volume plate. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. 81. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Molecular Devices Corporation 1311. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Quick View. Alves, Amelia K. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). 312 gain calibration failed. edu Contact Phone: 216-368-8962. Microscopy. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 1: SpectraMax Plus 384 components. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. , Sunnyvale, CA). Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. plates can be used in the SPECTRAmax PLUS 384. Awareness Technology Inc. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 0 1. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. The plate was covered and incubated for one hour at room temperature. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. 5. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Description 1. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Quick View. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMax Microplate Readers. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). 30. Streamline GLP/GMP compliance. Warranty. 3. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. This assay measures the two major sugars in wine, glucose and fructose. Supplier: Molecular Devices. 4. 314 low lamp level warning. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Service parts for these models are not being produced and are no longer in stock. 340 nm / 80 nm. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). #N207) LAL Reagent Water (Lonza cat. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. ). Near bottom. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Cell proliferation was measured using the MTT method. P. Immunofluorescence and Aggregation Analysis. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). 5. 8. (1993). The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The plate was covered and incubated for one hour at room temperature. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. MMP‑1 inhibition assay. The sensitivities of the hormones are Prolactin: 0. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Number of flashes. 315 can't find zero order. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. Values were determined according to a standard curve (log/log curve-fit). 00 USD. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. Call 1-800-745-2710. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Molecular Devices. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Description. MOLECULAR DEVICE M5. 4) was followed according to Vanacker et al. 2 M DEEMM were mixed in a microtube,. Details. They are for reference only. 05 ng/mL; progesterone: 2. Donor filter: 620 nm. 3. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Donor: 616/10 nm. These lab workhorses are used in drug discovery, research, bioassay validation, and. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Includes: SpectraMax 340PC384. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. Microplate Reader Shelf. the SpectraMax iD3 is a fantastic plate reader for a. D2. Unit powers. They are for reference only. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Inter-assay variation was prevented by testing all of the samples on the same day. SpectraMax Plus 384 measuring instruments pdf manual download. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. 5mM), and changes in OD 405 nm. SpectraMax 190 reads 96-well and strip-well plates. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). DNA extraction from blood meal ELISA homogenate. Briefly, cells were seeded into a. . Molecular Devices SpectraMax Gemini XS Microplate Reader. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. Data were expressed as the mean percent viable cell vs. One plate carrier adapter is provided with the instrument. Temperature Range: Ambient + 4 °C up to 45 °C. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Assay buffer was 50 m M Hepes, pH 7. 5. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. View the Molecular Devices website. 5. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Details. 5 g/L-EDTA 0. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 1. Microplate Readers. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Wound healing assay. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. 003 OD Stray light ≤ 0. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Wavelength(s) 340 to 850 nm; Add to Compare List. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . \r plates can be used in the SPECTRAmax PLUS 384. When necessary, samples were. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. reader (Molecular Devi ces). The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). 3: The cuvette chamber. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. Call 1-800-745-2710. 5 - 2. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. 4. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. Call 1-800-745-2710. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Also for: Spectramax 190, Spectramax 340pc384, Versamax. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. The loading equaled the mass of BSA per mass of particles. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Softmax PRO v5. SpectraMAX 340. 7 macrophages were obtained from ATCC (Manassas, VA). TGFα release was calculated as described in the Results. TGFα release was calculated as described in the Results. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). 9000-0756. To measure the cell biomass (g/L),. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. SpectraMax QuickDrop Micro-Volume Spectrophotometer. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. Each SpectraMax® 340 PC 384 Microplate Reader has an. 0 OD. SpectraMax Multi-Mode Microplate Readers.